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Isoelectric focusing (IEF) is a high-resolution technique where proteins are separated according to their isoelectric points within a continuous pH gradient. IEF is an electrophoretic method for separating proteins based on their isoelectric point. The isoelectric point is the pH at which the net charge of the protein is zero. With the presence of a pH gradient in the IEF technique, the protein will migrate to the position in the gradient where its charge is zero. Proteins with a positive net ch

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